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pgex sesn2  (Addgene inc)


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    Structured Review

    Addgene inc pgex sesn2
    Pgex Sesn2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+sesn2/pGEX-6p-1+Sesn2+(Plasmid+%2361873)/bio_rxiv__2025__06__09__658667-254-25-26
    Average 90 stars, based on 3 article reviews
    pgex sesn2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Expressing:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Construct:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Cloning:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.



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